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Mass Spectrometers

Q-Exactive HF-X (Thermo Scientific)

The HF-X is the latest offspring of the Q-Exactive family and offers rapid scanning and detection at very high resolution. Coupled to an EASY nLC 1200 UPLC system, the HF-X platform provides unmatched analytical performance particularly for data-independent acquisition approaches.

2 Q-Exactive Plus Orbitrap (Thermo Scientific)

Q-Exactive mass spectrometers combine quadruple precursor ion selection with high-resolution accurate-mass Orbitrap detection. Both Instruments in the facility are equipped with EASY nLc 1000 UPLC-Systems and column ovens allowing for the use column lengths up to 50 cm. This setup combines high resolution chromatography and the superior quality of the Q-Exactive instruments, enabling the quantitative analysis of complex biological samples in depth and with great confidence.

LTQ Orbitrap Discovery (Thermo Scientific)

The LTQ Orbitrap Discovery mass spectrometer (Thermo Scientific) is a hybrid system combining the LTQ XL linear ion trap mass spectrometer and the Orbitrap mass analyzer and is equipped with a HCD cell. The LTQ Orbitrap Discovery provides fast, sensitive and reliable identification and quantification of peptides/proteins as well as analyses of post-translational modifications. The LTQ Orbitrap Discovery is coupled to an EASY nLC II nano-LC system and equipped with a nano-ESI source.

Ultraflextreme MALDI-TOF-TOF (Bruker Daltonics)

The Ultraflextreme allows for fast determination of molecular masses of peptides and intact proteins. With this instrument In-Source Decay (ISD) fragmentation can be used for Top-down protein sequencing. This technique allows N- and C-terminal ladder sequencing up to 50 residues and is a valuable tool for quality control of purified proteins.

HCT ETD II PTM Discovery ESI-IT (Bruker Daltonics)

The HCT Ion Trap instrument is coupled to an EASY nLC II nano-LC system. It is equipped with the ETD option providing an alternative ion fragmentation technique. ETD is usually used in combination with CID in an alternating mode. The instrument is used for fast analysis of low complexity samples with moderate sensitivity.

The TripleTOF 6600 (SCIEX)

The TripleTOF 6600 is Q-TOF mass spectrometer that provides high resolution and mass accuracy on all MSn stages. The TripleTOF is coupled to an Eksigent nanoLC 2D system. It is specifically suited for shotgun and data-independent acquisition (DIA).

Dr. Christian Frese
Head of Proteomics Facility CECAD
Tel.  +49 221 478 84013
cfrese[at]uni-koeln.de

CECAD Research Institute
Universität zu Köln
Joseph-Stelzmann-Str. 26
50931 Köln

Dr. Christian Frese