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Imaging Facility

In line with the relocation to the new CECAD building at the end of 2013, the Imaging Facility was considerably expanded with the installation of new state-of-the-art microscopes and the recruitment of three new coworkers: one additional microscopist, Dr. Christian Jüngst, for assistance with and maintenance of the existing microscopes, one bioinformatician, Dr. Nikolay Kladt, for data analysis, and one technical assistant, Ira Hensen, for sample preparation and lab organization.

Services: The CECAD Imaging Facility offers a broad range of state-of-the-art microscopes. With the aforementioned addi-tional imaging equipment, it now has a pool of eight different microscopes that cover a broad range of microscopical applications such as FRAP, FLIM and laser ablation, but also ad-vanced techniques like super-resolution microscopy (STED and GSD) and multiphoton microscopy for the intravital imaging of living organisms. All of the systems can be booked online and used after extensive individual training. The facility also advises scientists on their imaging projects, set-up and method selection, and supports them in data analysis.

Scientific achievements: Apart from maintaining the existing microscopes, the facility is very interested in establishing and integrating new imaging techniques. This year, the team worked hand-in-hand with the users to successfully set up two different kinds of super-resolution microscopy (STED and GSD) as well as FLIM (Fluorescence Lifetime Imaging Microscopy) and intravital microscopy. Several custom-tailored analysis pipelines were written to offer users data analysis support.

Future plans: In the coming months, the imaging platform will be extended to include electron microscopy. It has applied for a new transmission electron microscope (TEM) and equipment for EM sample preparation. Two technicians trained in EM services, Beatrix Martiny and Jessica Hausmann, will join the team this month.
At present, the Imaging Facility is used by more than 300 scientists and over 80 research groups. But it is always open to new customers and extremely interested in cooperation projects in which it can actively participate.

Dr. Astrid Schauss
Head of Imaging Facility
Tel.  +49 221 478 840 27
aschauss[at]uni-koeln.de

CECAD Cologne / Imaging Facility
CECAD Forschungszentrum
Joseph-Stelzmann-Str. 26
50931 Köln

Astrid Schauss
Prices

We are charging for our services. Graduated charges apply for the different user groups (Members of CECAD, University staff, external scientists). Enquiries can be addressed to Dr. Astrid Schauss.

Figures
Figure 1
Figure 2

Figure 1: Visualization of the microtubule network of a human cell by classical confocal microscopy (left) and by STED microscopy (right). Stimulated emission depletion (STED) microscopy enables super resolution imaging by bypassing the diffraction limit of light microscopy (Christian Jüngst and Ira Hensen, AG Schauss).

Figure 2: Color coded 3D presentation of mouse retinal blood vessels stained with FITC-Dextran. Two-photon microscopy was performed using an upright laser scanning microscope equipped with an infrared
laser source which was tuned to 960 nm (Axel Witt, AG Kashkar).

Publications
  • Kondadi AK, Wang S, Montagner S, Kladt N, Korwitz A, Martinelli P, Herholz D, Baker MJ, Schauss AC, Langer T, Rugarli EI. (2014) Loss of the m-AAA protease subunit AFG3L2 causes mitochondrial transport defects and tau hyperphosphorylation. EMBO J. May 2;33(9):1011-26.
  • Rishmawi L, Pesch M, Juengst C, Schauss AC, Schrader A, Hülskamp M. (2014) Non-Cell-Autonomous Regulation of Root Hair Patterning Genes by WRKY75 in Arabidopsis. Plant Physiol. May;165(1):186-95.
  • Anand R, Wai T, Baker MJ, Kladt N, Schauss AC, Rugarli E, Langer T. (2014) The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. J Cell Biol. Mar 17;204(6):919-29.
  • Donovan C, Schauss A, Krämer R, Bramkamp M. (2013) Chromosome segregation impacts on cell growth and division site selection in Corynebacterium glutamicum. PLoS One. 2013;8(2):e55078.
  • Anton F., Fres J.M., Schauss A., Pinson B., Praefcke G.J., Langer T., Escobar-Henriques M. (2011) Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion. J Cell Sci Apr 1;124(Pt 7):1126-35.