Bioinformatic Facility
Peter Frommolt

CECAD Cologne / Bioinformatics
CECAD Forschungszentrum
Joseph-Stelzmann-Str. 26
D 50931 Köln
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Bioinformatics Facility

The Bioinformatics Facility assists biomedical research groups with computerised systems for processing molecular data of all kinds. The range of services provided by this CECAD facility covers four core areas:

  • Molecular data analysis
  • Data management
  • Software development
  • Advisory services

A key issue in the field of molecular data analysis is handling the enormous amounts of data that can be generated by current high-throughput technologies such as next-generation sequencing. For research groups handling a large volume of data, the Bioinformatics Facility offers support with data management and the setting up of appropriate databases. In close collaboration with CECAD research teams, it also develops software solutions to address specific scientific questions and, if required, will assist with any statistical or methodological problems relating to molecular data analysis.

Facts & Figures
  • Analysis pipelines for the processing of raw data from current experimental platforms
  • Use of cluster hardware for massive parallel processing in collaboration with the University Computing Center (RRZK)
  • Expertise in the field of bioinformatics and statistics
Figure 1: Scheme of data evaluation for next-generation sequencing at the Bioinformatics Facility.  A database stores meta-information for analysis control and results, while the actual data processing is carried out on a computer cluster at the University Computing Center (RRZK). The exported data can then be retrieved via a web server. The system is currently undergoing further development.
Fig. 1
Figure 2: In conjunction with a CECAD research group, the Bioinformatics Facility is developing the software program Wormpath for the analysis of genetic networks in the nematode C. elegans. The figure shows a diagram of a signal pathway in the worm, which is upregulated in response to stress. The software tool is organized as a web service and can be accessed via the link: http://x4440.ccg.uni-koeln.de/wormpath.
Fig. 2
Figure 2

In conjunction with a CECAD research group, the Bioinformatics Facility is developing the software program Wormpath for the analysis of genetic networks in the nematode C. elegans. The figure shows a diagram of a signal pathway in the worm, which is upregulated in response to stress. The software tool is organized as a web service and can be accessed via the link: x4440.ccg.uni-koeln.de/wormpath.

Publications
  • Meimaridou E, Kowalczyk J, Guasti L, Hughes CR, Wagner F, Frommolt P, Nürnberg P, Mann NP, Banerjee R, Saka HN, Chapple JP, King PJ, Clark AJ, Metherell LA (2012) Mutations in NNT encoding nicotinamide nucleotide transhydrogenase cause familial glucocorticoid deficiency.
    Nat Genet; 44(7): 740-2
  • Frommolt P, Abdallah AT, Altmüller J, Motameny S, Thiele H, Becker C, Stemshorn K, Fischer M, Freilinger T, Nürnberg P (2012) Assessing the target enrichment performance in next-generation sequencing.
    Hum Mutat; 33(4): 635-41
  • Frommolt P, Thomas RK (2008) Standardized high-throughput evaluation of cell-based compound screens.
    BMC Bioinformatics, 9: 475
Picture credit

Header Picture: Courtesy of Dieter Both, Bull GmbH.